Biotransformation of Chitin to Chitosan using Bacillus cereus JCM44 isolated from Kanyakumari, India and its Characterization
نویسندگان
چکیده
Bacillus cereus JCM44 was isolated from the soil samples collected Kanyakumari district of Tamil Nadu, using a minimal agar medium. It characterized by its ability to biotransform chitin into chitosan at 30°C for 48 h. The Chitosan (CS) recovered 0.1N Sodium hydroxide and 2% acetic acid. confirmed iodine solution with dark purple colouration qualitatively identified acetone ethanol. CS formation has been X-ray diffraction (XRD) Fourier Transform Infrared Spectroscopy (FTIR). Scanning Electron Microscopy (SEM) analysis elucidated an average size 349 nm 1µm morphology non-porous, smooth membrane irregular shape crystallite. Zeta potential study bacterially synthesized showed their excellent stability surface charge -2.28 mV. Bacterially Synthesized (BSC), polydispersity index (PDI) value 0.626 indicates aggregation particles. peaks higher wavenumber confirm blend in (BCS) OH NH denoted. XRD BSC detected crystallinity when compared commercial chitosan.
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ژورنال
عنوان ژورنال: Journal of Pure and Applied Microbiology
سال: 2023
ISSN: ['2581-690X', '0973-7510']
DOI: https://doi.org/10.22207/jpam.17.1.02